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欖李、豬籠草及愛玉之天然物及其應用開發 =Natural Product...

余思穎

欖李、豬籠草及愛玉之天然物及其應用開發 =Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	欖李、豬籠草及愛玉之天然物及其應用開發 =/ 余思穎
Reminder of title:	Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development /
remainder title:	Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development
Author:	余思穎
Published:	[高雄市]: [撰者], : 民112,
Description:	148葉 :圖 ; : 30公分;
Notes:	指導教授: 張芳榮.
基督教聖經之智慧書導讀 :	藥物開發的流程中，常藉由天然物研究尋找適合作為先導化合物的天然物，先導化合物經由一系列分離純化、成分鑑定、成分分析以及活性測試等研究。對於，通常需要使用全合成或半合成方法增加其製備量，以進一步進行構效關係的優化。現今，各種技術的綜合應用已成為天然藥物開發的基礎。為了深入了解欖李、豬籠草及愛玉萃取物的化學成分及生物活性之探討，分別採集欖李枝葉、購買一種豬籠草全株及愛玉枝葉，並與辜嚴倬雲植物保種中心合作，收集60種不同種之豬籠草作為研究材料，進行進一步研究。本研究進行了初步的活性篩選，發現它們各具有下列生物活性：欖李之甲醇萃取物則具有抑制血管新生之活性；一種豬籠草的甲醇萃取物可抑制彈性蛋白酶釋放，具有抗發炎活性；愛玉枝葉甲醇萃取物具活化Nrf2之活性。因此，本研究運用活性導引分離法、分子網絡、質譜分析等，針對具潛力的題材進行活性天然物的分離、純化及鑑定，並配合HPLC-PDA、分光光度計及LC-MS/MS等儀器進行各項分析實驗，並且針對部分研究題材進行後續的應用。搭配上述方法進行研究，自欖李中發現具有抑制血管新生的新化合物racelactone A;豬籠草的萘醌類成分被發現對抑制人類肝癌細胞株、人類三陰性乳癌細胞株以及人類非小細胞肺癌細胞株及抗發炎活性具有相關性;愛玉枝葉則是經由文獻、電腦模擬及分析實驗，確認其主要的成分類型具有但不限於抑制SARS-CoV2病毒活性之潛力並取得相關專利。.
Online resource:	電子資源
[NT 15000934]:	(平裝)

欖李、豬籠草及愛玉之天然物及其應用開發 =Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development /
余思穎

欖李、豬籠草及愛玉之天然物及其應用開發 =Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development /Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development余思穎 - [高雄市]: [撰者], 民112 - 148葉 :圖 ;30公分

指導教授: 張芳榮.

博士論文--高雄醫學大學天然藥物研究所博士班.

參考書目：葉.

Table of Contents

藥物開發的流程中，常藉由天然物研究尋找適合作為先導化合物的天然物，先導化合物經由一系列分離純化、成分鑑定、成分分析以及活性測試等研究。對於，通常需要使用全合成或半合成方法增加其製備量，以進一步進行構效關係的優化。現今，各種技術的綜合應用已成為天然藥物開發的基礎。為了深入了解欖李、豬籠草及愛玉萃取物的化學成分及生物活性之探討，分別採集欖李枝葉、購買一種豬籠草全株及愛玉枝葉，並與辜嚴倬雲植物保種中心合作，收集60種不同種之豬籠草作為研究材料，進行進一步研究。本研究進行了初步的活性篩選，發現它們各具有下列生物活性：欖李之甲醇萃取物則具有抑制血管新生之活性；一種豬籠草的甲醇萃取物可抑制彈性蛋白酶釋放，具有抗發炎活性；愛玉枝葉甲醇萃取物具活化Nrf2之活性。因此，本研究運用活性導引分離法、分子網絡、質譜分析等，針對具潛力的題材進行活性天然物的分離、純化及鑑定，並配合HPLC-PDA、分光光度計及LC-MS/MS等儀器進行各項分析實驗，並且針對部分研究題材進行後續的應用。搭配上述方法進行研究，自欖李中發現具有抑制血管新生的新化合物racelactone A;豬籠草的萘醌類成分被發現對抑制人類肝癌細胞株、人類三陰性乳癌細胞株以及人類非小細胞肺癌細胞株及抗發炎活性具有相關性;愛玉枝葉則是經由文獻、電腦模擬及分析實驗，確認其主要的成分類型具有但不限於抑制SARS-CoV2病毒活性之潛力並取得相關專利。.

(平裝)Subjects--Index Terms:

欖李

欖李、豬籠草及愛玉之天然物及其應用開發 =Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development /
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245 1 0 $a 欖李、豬籠草及愛玉之天然物及其應用開發 = $b Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development / $c 余思穎
246 1 1 $a Natural Products of Lumnitzera racemosa, Nepenthes, and Ficus pumila var. awkeotsang and Their Application Development
260 # $a [高雄市]: $b [撰者], $c 民112
300 $a 148葉 : $b 圖 ; $c 30公分
500 $a 指導教授: 張芳榮.
500 $a 學年度: 111.
502 $a 博士論文--高雄醫學大學天然藥物研究所博士班.
504 $a 參考書目：葉.
505 0 # $a Table of Contents
520 3 $a 藥物開發的流程中，常藉由天然物研究尋找適合作為先導化合物的天然物，先導化合物經由一系列分離純化、成分鑑定、成分分析以及活性測試等研究。對於，通常需要使用全合成或半合成方法增加其製備量，以進一步進行構效關係的優化。現今，各種技術的綜合應用已成為天然藥物開發的基礎。為了深入了解欖李、豬籠草及愛玉萃取物的化學成分及生物活性之探討，分別採集欖李枝葉、購買一種豬籠草全株及愛玉枝葉，並與辜嚴倬雲植物保種中心合作，收集60種不同種之豬籠草作為研究材料，進行進一步研究。本研究進行了初步的活性篩選，發現它們各具有下列生物活性：欖李之甲醇萃取物則具有抑制血管新生之活性；一種豬籠草的甲醇萃取物可抑制彈性蛋白酶釋放，具有抗發炎活性；愛玉枝葉甲醇萃取物具活化Nrf2之活性。因此，本研究運用活性導引分離法、分子網絡、質譜分析等，針對具潛力的題材進行活性天然物的分離、純化及鑑定，並配合HPLC-PDA、分光光度計及LC-MS/MS等儀器進行各項分析實驗，並且針對部分研究題材進行後續的應用。搭配上述方法進行研究，自欖李中發現具有抑制血管新生的新化合物racelactone A;豬籠草的萘醌類成分被發現對抑制人類肝癌細胞株、人類三陰性乳癌細胞株以及人類非小細胞肺癌細胞株及抗發炎活性具有相關性;愛玉枝葉則是經由文獻、電腦模擬及分析實驗，確認其主要的成分類型具有但不限於抑制SARS-CoV2病毒活性之潛力並取得相關專利。.
520 3 $a The drug development process involves multiple steps. Among them, the study of natural compounds as important lead compound sources requires a series of research, including isolation, purification, component analysis, and activity testing. In addition, total synthesis or semi-synthesis methods is typically necessary to increase the quantity of lead compounds using for further studying their structure-activity relationship. Moreover, the integrated application of various latest technologies has become the foundation of natural drug development. Branches and leaves of Lumnitzera racemosa, whole plants of Nepenthes sp., and branches and leaves of Ficus pumila var. awkeotsang were collected to gain a deeper understanding of the chemical composition and biological activities of their extracts. In collaboration with the Dr. Cecilia Koo Botanic Conservation Center, we collected 60 different species of Nepenthes as research materials for further investigation. The preliminary activity screening found the following biological activities: the methanolic extract of L. racemosa exhibited inhibitory activity against angiogenesis; the methanol extract of Nepenthes sp. showed inhibition of elastase release, indicating anti-inflammatory activity; the methanol extract of F. pumila var. awkeotsang branches and leaves exhibited Nrf2 activation activity. Furthermore, we employed techniques such as activity-guided isolation, molecular networking, and mass spectrometry for the isolation, purification, and identification of potentially active natural compounds. Various analytical experiments were conducted using instruments such as HPLC-PDA, spectrophotometer, and LC-MS/MS. The subsequent applications were carried out for certain research subjects. The further study utilized activity-guided isolation, compound analysis, and component analysis, supplemented by computer simulation of molecular docking and global natural product molecular networking for auxiliary analysis. A new compound called racelactone A with inhibitory activity against angiogenesis was discovered from L. racemosa. Naphthoquinone components from Nepenthes were found to be relevant to the inhibition of human liver cancer cells, human triple-negative breast cancer cells, and human non-small cell lung cancer cells, as well as anti-inflammatory activity. Moreover, the main component types of F. pumila var. awkeotsang were confirmed through literature, computer simulation, and analysis experiments to have potential, but not limited to, the inhibition of SARS-CoV-2 virus activity. Several patents related to the research findings were obtained..
563 $a (平裝)
653 # # $a 欖李 $a 豬籠草 $a 愛玉 $a 分子網絡.
653 # # $a Lumnitzera racemosa $a Nepenthes $a Ficus pumila var. awkeotsang $a molecular networking.
856 7 # $u https://handle.ncl.edu.tw/11296/rgur37 $z 電子資源 $2 http