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Culture of animal cells :a manual of...

Freshney, R. Ian.

Culture of animal cells :a manual of basic technique and specialized applications /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Culture of animal cells :/ R. Ian Freshney.
其他題名:	a manual of basic technique and specialized applications /
作者:	Freshney, R. Ian.
出版者:	Hoboken, N.J. :Wiley-Blackwell, : c2010.,
版本:	6th ed.
面頁冊數:	xxxi, 732 p., [28] p. of plates :ill. (some col.) ; : 29 cm.;
標題:	Culture Techniques - methods. -
ISBN:	9780470528129 (hard) :

Culture of animal cells :a manual of basic technique and specialized applications /
Freshney, R. Ian.

Culture of animal cells :a manual of basic technique and specialized applications /R. Ian Freshney. - 6th ed. - Hoboken, N.J. :Wiley-Blackwell,c2010. - xxxi, 732 p., [28] p. of plates :ill. (some col.) ;29 cm.

Includes bibliographical references and index.

Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Germ Cells, and Amniocytes -- Culture of Tumor Cells -- Three-Dimensional Culture -- Scale-up and Automation -- Specialized Techniques -- Training Programs -- Problem Solving -- In Conclusion

ISBN: 9780470528129 (hard) :NT2999

LCCN: 2010007042

Nat. Bib. No.: GBB065043bnb

Nat. Bib. Agency Control No.: 015562264UkSubjects--Topical Terms:

335843
Culture Techniques
--methods.

LC Class. No.: QH585.2 / .F74 2010

Dewey Class. No.: 571.6/381

National Library of Medicine Call No.: 2010 L-707

Culture of animal cells :a manual of basic technique and specialized applications /
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245 1 0 $a Culture of animal cells : $b a manual of basic technique and specialized applications / $c R. Ian Freshney.
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505 0 $a Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Germ Cells, and Amniocytes -- Culture of Tumor Cells -- Three-Dimensional Culture -- Scale-up and Automation -- Specialized Techniques -- Training Programs -- Problem Solving -- In Conclusion
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