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以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= Sura...

黃士豪

以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/

Record Type:	Language materials, printed : Monograph/item
Title/Author:	以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= / 黃士豪
Reminder of title:	Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/
remainder title:	Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression
Author:	黃士豪
Published:	[高雄市]: [撰者], : 民111,
Description:	58葉: 圖; : 30公分;
Notes:	指導教授: 盧政昌.
基督教聖經之智慧書導讀 :	研究重點旋轉肌肌腱病變是肩部最常見的疾病，常造成患者肩部疼痛、功能下降甚至導致嚴重性關節炎。近期的研究顯示發炎反應在對於旋轉肌病變病程中扮演重要角色。目前治療旋轉肌病變藥物非常有限，尋找有效率的藥物是必要的。蘇拉明(Suramin) 是一種 FDA 核准的古老藥物，許多研究發現蘇拉明具有抗凋亡、抗纖維化、抗炎和抗氧化能力。然而，蘇拉明對肌腱細胞和旋轉肌腱病變的影響仍不清楚。方法或程序在關節鏡下採集接受旋轉肌腱斷裂修補手術病人之旋轉肌腱斷端組織進行細胞培養，以MMT試驗評估蘇拉明對肌腱細胞的毒性及活性的影響。Ki-67、PCNA表現和細胞計數用於評估蘇拉明處理後的細胞增殖情況。西方點墨法和即時聚合酶連鎖反應用於測量受蘇拉明影響的肌腱蛋白質及基因因子表現量。細胞遷移測定用於測量肌腱細胞受蘇拉明作用後的遷移性。對於評估蘇拉明對發炎反應及巨噬細胞M1極化的影響，用蘇拉明處理THP-1巨噬細胞再誘導 M1 巨噬細胞極化。結果本研究發現蘇拉明可促進旋轉肌肌腱細胞存活及細胞增殖速率，並促進肌腱細胞產生較多的膠原蛋白1及3a。蘇拉明促進肌腱細胞分泌促血管生成因子VEGF分泌。蘇拉明增加肌腱細胞遷移能力。蘇拉明顯著降低THP-1在發炎細胞因子的產生及M1巨嗜細胞極化。這些結果顯示蘇拉明可以有效增加旋轉肌肌腱細胞活性並抑制巨噬細胞發炎反應的產生。它可以作為治療旋轉肌腱病變及促進肌腱修復的潛在藥物。.
Online resource:	電子資源
[NT 15000934]:	(平裝)

以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/
黃士豪

以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/ Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression黃士豪 - [高雄市]: [撰者], 民111 - 58葉: 圖; 30公分

指導教授: 盧政昌.

碩士論文--高雄醫學大學臨床醫學研究所.

參考書目：葉.

目錄

研究重點旋轉肌肌腱病變是肩部最常見的疾病，常造成患者肩部疼痛、功能下降甚至導致嚴重性關節炎。近期的研究顯示發炎反應在對於旋轉肌病變病程中扮演重要角色。目前治療旋轉肌病變藥物非常有限，尋找有效率的藥物是必要的。蘇拉明(Suramin) 是一種 FDA 核准的古老藥物，許多研究發現蘇拉明具有抗凋亡、抗纖維化、抗炎和抗氧化能力。然而，蘇拉明對肌腱細胞和旋轉肌腱病變的影響仍不清楚。方法或程序在關節鏡下採集接受旋轉肌腱斷裂修補手術病人之旋轉肌腱斷端組織進行細胞培養，以MMT試驗評估蘇拉明對肌腱細胞的毒性及活性的影響。Ki-67、PCNA表現和細胞計數用於評估蘇拉明處理後的細胞增殖情況。西方點墨法和即時聚合酶連鎖反應用於測量受蘇拉明影響的肌腱蛋白質及基因因子表現量。細胞遷移測定用於測量肌腱細胞受蘇拉明作用後的遷移性。對於評估蘇拉明對發炎反應及巨噬細胞M1極化的影響，用蘇拉明處理THP-1巨噬細胞再誘導 M1 巨噬細胞極化。結果本研究發現蘇拉明可促進旋轉肌肌腱細胞存活及細胞增殖速率，並促進肌腱細胞產生較多的膠原蛋白1及3a。蘇拉明促進肌腱細胞分泌促血管生成因子VEGF分泌。蘇拉明增加肌腱細胞遷移能力。蘇拉明顯著降低THP-1在發炎細胞因子的產生及M1巨嗜細胞極化。這些結果顯示蘇拉明可以有效增加旋轉肌肌腱細胞活性並抑制巨噬細胞發炎反應的產生。它可以作為治療旋轉肌腱病變及促進肌腱修復的潛在藥物。.

(平裝)Subjects--Index Terms:

蘇拉明

以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/
LDR:04804cam a2200229 i 4500 001 355292
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035 $a (THES)110KMC00521008
100 1 $a 黃士豪 $3 488409
245 1 0 $a 以蘇拉明治療來增強受傷的旋轉肌肌腱細胞之增生和膠原蛋白的表現= $b Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression/ $c 黃士豪
246 1 1 $a Suramin treatment enhances the injured rotator cuff tenocyte's proliferation, migration, and collagen expression
260 # $a [高雄市]: $b [撰者], $c 民111
300 $a 58葉: $b 圖; $c 30公分
500 $a 指導教授: 盧政昌.
500 $a 學年度: 110.
502 $a 碩士論文--高雄醫學大學臨床醫學研究所.
504 $a 參考書目：葉.
505 0 # $a 目錄
520 3 $a 研究重點旋轉肌肌腱病變是肩部最常見的疾病，常造成患者肩部疼痛、功能下降甚至導致嚴重性關節炎。近期的研究顯示發炎反應在對於旋轉肌病變病程中扮演重要角色。目前治療旋轉肌病變藥物非常有限，尋找有效率的藥物是必要的。蘇拉明(Suramin) 是一種 FDA 核准的古老藥物，許多研究發現蘇拉明具有抗凋亡、抗纖維化、抗炎和抗氧化能力。然而，蘇拉明對肌腱細胞和旋轉肌腱病變的影響仍不清楚。方法或程序在關節鏡下採集接受旋轉肌腱斷裂修補手術病人之旋轉肌腱斷端組織進行細胞培養，以MMT試驗評估蘇拉明對肌腱細胞的毒性及活性的影響。Ki-67、PCNA表現和細胞計數用於評估蘇拉明處理後的細胞增殖情況。西方點墨法和即時聚合酶連鎖反應用於測量受蘇拉明影響的肌腱蛋白質及基因因子表現量。細胞遷移測定用於測量肌腱細胞受蘇拉明作用後的遷移性。對於評估蘇拉明對發炎反應及巨噬細胞M1極化的影響，用蘇拉明處理THP-1巨噬細胞再誘導 M1 巨噬細胞極化。結果本研究發現蘇拉明可促進旋轉肌肌腱細胞存活及細胞增殖速率，並促進肌腱細胞產生較多的膠原蛋白1及3a。蘇拉明促進肌腱細胞分泌促血管生成因子VEGF分泌。蘇拉明增加肌腱細胞遷移能力。蘇拉明顯著降低THP-1在發炎細胞因子的產生及M1巨嗜細胞極化。這些結果顯示蘇拉明可以有效增加旋轉肌肌腱細胞活性並抑制巨噬細胞發炎反應的產生。它可以作為治療旋轉肌腱病變及促進肌腱修復的潛在藥物。.
520 3 $a Background Rotator cuff (RC) tendinopathy is the most common shoulder disease. Currently, there are limited medication treatments for RC tendinopathy. It is essential to find effective medication for RC tendinopathy. Suramin previously used to treat African sleeping sickness possess the capability of anti-apoptoic, anti-fibrotic, anti-inflammation and anti-oxidative stress. However, the effects of suramin on tenocytes and RC tendinopathy need to investigate. Materials and Methods Tenocytes were isolated form injury rotator tendon tissues in patients received arthroscopic rotator cuff tendon repair. MMT assay was used to evaluate the toxicity of suramin to tenocytes. Ki-67, PCNA and cell count were used to evaluate cell proliferation after suramin treatment. Western blot and RT-PCR were used to measure the protein and gene expression of tenogenic factors in injured RC tenocytes treated by suramin. Migration assay was used to measure activity of tenocytes. THP-1 cell cultured with suramin prior to M1 macrophage induction was used to estimate the effect of suramin on anti-inflammatory response and M1 macrophage polarization. Results In this study, suramin significantly increased cell viability and proliferation rate of RC tenocytes. After suramin treatment, the RC tenocytes expressed significantly higher protein and mRNA of COL1A , COL3A and VEGF. In addition, suramin treatment significantly increase the migration ability of rotator cuff remnant tenocytes compared to that without suramin treatment. For the anti-inflammatory effect, suramin effectively decreased inflammatory cytokines release and M1 macrophage polarization in THP-1 cells. Conclusion These results strongly suggest that suramin treatment could enhance the cell activity and proliferation of injured RC tenocytes. Besides, suarmin could diminish the inflammation and M1 macrophage polarization in THP-1 cell. Suramin could serve as a potential medication for treating rotator cuff partial tear or augmenting the healing after RC repair..
563 $a (平裝)
653 # # $a 蘇拉明 $a 旋轉肌腱病變 $a 發炎反應 $a 巨噬細胞.
653 # # $a suramin $a srotator cuff tendinopathy $a inflammation $a macrophage polarization.
856 7 # $u https://handle.ncl.edu.tw/11296/my6bf3 $z 電子資源 $2 http